DNA Cloning 2: A Practical Approach - D. M. Glover

Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications.
These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series.
Together they will offer a complete guide to the major techniques required by modern molecularbiology laboratories.