Gene Transcription - B. David Hames

Understanding the mechanisms and control of gene transcription is said to be one of the central goals for a large proportion of molecular biologists currently involved in research. This book concentrates on RNA polymerase II transcription of eukaryotic protein-coding systems, but many of the approaches and techniques described apply equally well to the study of systems involving RNA polymerases I and III.;This book covers the major current procedures and approaches in this field, including not only the analysis of transcription "in vitro" and "in vivo", but also the identification, purification and characterization of transcription factors and their interaction with specific DNA target sites.;It is written by experts and specifically designed for all researchers intent on studying eukaryotic gene expression. Its mix of background information, advice on experimental approaches, detailed practical protocols, typical results, help with troubleshooting and reference appendices make it a guide for the newcomer to the field, and a source of new approaches and techniques for more experienced researchers.;David Hames is one of the series editors of the "Practical Approach" series. Other titles in the "PA" series include "Transcription and Translation", "Nucleic Acid Hybridization", "Gel Electrophoresis of Proteins" and "Gel Electrophoresis of Nucleic Acids".